Real-time bacterial microcolony Automated counting - CountThings By Camera
Colony counting, often performed manually using the light box and pen method, demands a very high level of concentration by the microbiologist. As a way to manage this error-prone and time-consuming process, researchers resort to analyzing only parts of a plate to estimate whole plate numbers, introducing inaccuracies in the data.
To simplify the colony counting process, scientists often work with colored bacterial colonies by either using chromogenic media or by staining the colonies. Although easier to visualize compared to uncolored bacteria, obtaining accurate colored counts relies on the microbiologist having perfect color vision. As the brain interprets colors differently, there can be discrepancies in the colony counts when multiple scientists work on the same project.
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